Echinatin Inhibits Oxidative Stress and Inflammatory Processes in Trophoblast Cells by Inhibiting TLR4-MyD88-NF-κB Pathway in Preeclampsia

Figure 3

Echinatin induces viability and migration of HTR-8/SVneo cells through the TLR4-MyD88-NF-κB pathway. (a) After a 24 h exposure of HTR-8/SVneo cells to echinatin, the Western blotting technique was used to assess the levels of TLR4, MyD88, NF-κB, p-NF-κB, IkB, and p-IkB protein expressions. (b)–(e) After treating HTR-8/SVneo cells overexpressing TLR4 with echinatin for 24 h. (b) The expression of TLR4, MyD88, NF-κB, p-NF-κB, IkB, and p-IkB was detected using Western blotting. (c) Cell viability of HTR-8/SVneo cells was assessed using the EdU assay. (d) Cell viability of HTR-8/SVneo cells was assessed using the CCK-8 assay. (e) Cell migration of HTR-8/SVneo cells was analyzed using the Transwell assay. Mean ± SD; , , and .

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