Echinatin Inhibits Oxidative Stress and Inflammatory Processes in Trophoblast Cells by Inhibiting TLR4-MyD88-NF-κB Pathway in Preeclampsia

Figure 4

Echinatin alleviates H₂O₂-induced inflammation, oxidative stress, and cell apoptosis through the TLR4-MyD88-NF-κB pathway. (a)–(h) Treating HTR-8/SVneo cells with 200 μM H₂O₂ or 40 μM echinatin in combination with overexpressing TLR4. (a) ROS generation was measured using the DCFH-DA method. Intracellular levels of MDA (b), SOD activity (c), and GSH-Px activity (d). (e)–(g) ELISA experiments were performed to measure the levels of IL-18 (e), TNF-α (f), and IL-6 (g). (h) Flow cytometry was used to detect the apoptosis rate of the cells. Mean ± SD; , , and .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)