Detection of Elevated Signaling Amino Acids in Human Diabetic Vitreous by Rapid Capillary Electrophoresis

Lu, Miao-Jen; Pulido, Jose S.; McCannel, Colin A.; Pulido, Jose E.; Hatfield, R. Mark; Dundervill, Robert F.; Shippy, Scott A.

doi:https://doi.org/10.1155/2007/39765

Journal of Diabetes Research

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Diabetic Retinopathy: From Pathogenesis to Treatment

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Research Article | Open Access

Volume 2007 | Article ID 039765 | https://doi.org/10.1155/2007/39765

Detection of Elevated Signaling Amino Acids in Human Diabetic Vitreous by Rapid Capillary Electrophoresis

Miao-Jen Lu,¹Jose S. Pulido,²Colin A. McCannel,²Jose E. Pulido,²R. Mark Hatfield,³Robert F. Dundervill,³and Scott A. Shippy¹

Academic Editor: Subrata Chakrabarti

Received30 Jan 2007

Revised27 Mar 2007

Accepted19 Apr 2007

Published25 Jun 2007

Abstract

Elevated glutamate is implicated in the pathology of PDR. The ability to rapidly assess the glutamate and amino acid content of vitreous provides a more complete picture of the chemical changes occurring at the diabetic retina and may lead to a better understanding of the pathology of PDR. Vitreous humor was collected following vitrectomies of patients with PDR and control conditions of macular hole or epiretinal membrane. A capillary electrophoresis method was developed to quantify glutamate and arginine. The analysis is relatively fast (<6 minutes) and utilizes a poly(ethylene)oxide and sodium dodecylsulfate run buffer. Both amino acid levels show significant increases in PDR patients versus controls and are comparable to other reports. The levels of vitreal glutamate vary inversely with the degree of observed hemorrhage. The results demonstrate a rapid method for assessment of a number of amino acids to characterize the chemical changes at the diabetic retina to better understand tissue changes and potentially identify new treatments.

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Copyright © 2007 Miao-Jen Lu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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