Pancreatic Beta-Cell Purification by Altering FAD and NAD(P)H Metabolism

Table 1

(a) Composition of culture media used for beta-cell isolation. (b) Concentration of amino acids which are known to stimulate beta-cell insulin secretion, in the culture media used for beta-cell isolation.

(a)

	D-Glucose (mM)	Amino acid content (mg/L)	FAD (mg/L)	Riboflavin (mg/L)
MEM	5.5	553	0	0.1
RPMI	11	1010	0	0.2
CMRL	5.5	860	1	0.01
DMEM-F12	17	553	0	0.219

(b)

	L-Glutamine	L-Alanine	L-Leucine	L-Arginine-HCL
	mM (mg/L)	mM (mg/L)	mM (mg/L)	mM (mg/L)
MEM	0 (0)	0 (0)	0.4 (52.0)	0.6 (126)
RPMI	2.0 (300)	0 (0)	0.4 (50)	1.1 (240)
CMRL	0 (0)	0.3 (25)	0.5 (60)	0.3 (70)
DMEM-F12	2.5 (365)	0.05 (4.45)	0.5 (59.05)	0.7 (147.50)