Murine Pancreatic Beta TC3 Cells Show Greater 2^′,5^′-Oligoadenylate Synthetase (2^′5^′AS) Antiviral Enzyme Activity and Apoptosis Following IFN-𝛼 or Poly(I:C) Treatment than Pancreatic Alpha TC3 Cells

Figure 4

Enzyme activity of PKR in non-stimulated and IFN-α stimulated beta TC3 and alpha TC3 cells. Beta TC3 and alpha TC3 cells were incubated with or without addition of 500 U/mL of IFN-α into the medium for 24 hours in three independent experiments. The lysates were subjected to an autophosphorylation assay with 𝛾³²P-ATP. Incubation time was 1 hour. Proteins were fractionated by 12% SDS-PAGE and radioactive bands were visualized by autoradiography. After the gels were dried, the protein bands were cut and the radioactivity was quantitated in a scintillation counter.