Murine Pancreatic Beta TC3 Cells Show Greater 2^′,5^′-Oligoadenylate Synthetase (2^′5^′AS) Antiviral Enzyme Activity and Apoptosis Following IFN-𝛼 or Poly(I:C) Treatment than Pancreatic Alpha TC3 Cells

Figure 8

FACS analysis of apoptosis induction in beta TC3 and alpha TC3 cells after transfection of mouse IFN-α4-containing vector and poly(I:C). Beta TC3 and alpha TC3 cells were transiently transfected with pcDNA3.1-mIFN-α4, poly(I:C), and pcDNA3.1-mIFN-α4 plus poly(I:C), respectively. pcDNA3.1 empty vector was used as a negative control. After 24 hours, the cell samples were stained with the Annexin-V-PE and 7-AAD kit, and induction of apoptosis was assayed by FACS analysis. (a) Beta TC3 cells; (b) alpha TC3 cells; (c) the percentage of apoptosis of induction in beta TC3 and alpha TC3 cells after transfection. ^∗𝑃<.05, ^∗∗𝑃<.005, ^∗∗∗𝑃<.0001 (transfected group versus control, 𝑛=3).

(a) 𝛽TC3

(b) 𝛼TC3

(c)