NF-κB-Inducing Kinase Increases Renal Tubule Epithelial Inflammation Associated with Diabetes
Figure 2
NIK overexpression in HK-2 cells increases noncanonical NF-κB pathway activation: HK-2 cells were transfected with the eukaryotic expression vectors pCDNA-MycNIK (NIKwt) or pCDNA-MycNIK dominant negative (NIKdn), encoding NIK Thr559Phe site mutant or empty vector (ev) plasmid DNA (pCDNA). (a) Representative Western blot showing expression of NIKwt and NIKdn transfected at the same plasmid concentration; (b) Western blot showing nuclear RelB on the left and quantification of 4 separate experiments on the right; (c) Representative Western blot showing cytosolic and nuclear p100 and p52 on the left and quantification of nuclear p52 from 5 separate experiments on the right. P100 is predominantly cytosolic and despite the increase in nuclear p52, it did not change in abundance. (d) Representative Western blot showing cytosolic and nuclear RelA on the left and quantification of nuclear RelA from 4 separate experiments on the right. Bar graph data are presented as mean ± SE; Student’s t-test versus empty vector control (ev): *.
