Increased Expression of the Tail-Anchored Membrane Protein SLMAP in Adipose Tissue from Type 2 Tally Ho Diabetic Mice
Figure 2
Expression of SLMAP isoforms in adipose tissue in Tally Ho mice. Adipose tissue was isolated and analyzed by immunoblotting with anti-SLMAP (a). Anti-SLMAP immunoreactive proteins are depicted by arrows, and the positions of migration of the molecular weight standards are shown as numbers in (kilodaltons). The expressions of the 90, 45, and 35 kDa SLMAP from adipose tissue in both NG and HG Tally Ho mice were quantified by densitometry and are shown as bar graph (b). Real-time PCR for SLMAP message normalized to β-actin from both NG and HG Tally Ho mice (c). Subcellular membrane fractions Cyto (cytosolic fraction) and Mem (membrane fraction) from adipose tissue in both NG and HG Tally Ho mice were analyzed by immunoblotting with anti-SLMAP (d). The expression of the 35 and 45 kDa isoforms of SLMAP from membrane fraction was quantified by densitometry and shown as bar graph (d,e). The data are expressed as mean ± SEM. 4 to 6 mice were included in each group. *Significantly different () .
