Involvement of F-Actin in Chaperonin-Containing t-Complex 1 Beta Regulating Mouse Mesangial Cell Functions in a Glucose-Induction Cell Model
Figure 1
Optimal ratio between transfection reagent (TR) and volume of siRNA (TR-to-siRNA) for a low CCT2 mouse mesangial cell (mMC) model. The volume of TR was fixed at 6 ul, and transfection was performed with varying volumes of CCT2 siRNA. Representative plots show (a) western blotting used for the expression of CCT2 in mMC treated by TR only and TR plus CCT2siRNA at the ratios of 6 : 2, 6 : 4, and 6 : 6 and (b) the normalized CCT2 expression against GAPDH for above groups, in which group 6 : 2 displays an ideal suppressive effect on CCT2 and was chosen for subsequent experiments. versus group TR.
