Involvement of F-Actin in Chaperonin-Containing t-Complex 1 Beta Regulating Mouse Mesangial Cell Functions in a Glucose-Induction Cell Model
Figure 2
CCT2 and F/G-actin ratios in the CCT2 cell model with and without high glucose induction. The TR-to-siRNA ratio of 6 : 2 was used for the CCT2 knockdown experiment with evaluation of F/G-actin ratio on six designated groups. Representative plots show (a1) western blotting CCT2 expression and (a2) the normalized CCT2 expression against GAPDH for the six groups, in which induction of CCT2 expression was observed with high glucose group of transfection reagent (TR pH) and scramble siRNA (CsiRNA pH) and reduction of CCT2 expression was with normal glucose group (CCT2siRNA pN) and high glucose group (CCT2siRNA pH) of siRNA as compared to control group (TR pN). Denotes versus group TR pN. Under the same conditions, changes of the F/G-actin ratio were investigated with four subtly designated groups. As representative plot (b1) shows the expression of F- and G-actins responding to the four conditions, and plot (b2) shows the ratios of F-actin and G-actin expressed in the four conditions. Compared to control group CsiRNA pN, the decrease of F/G-actin ratio was observed for all experimental groups .
