Involvement of F-Actin in Chaperonin-Containing t-Complex 1 Beta Regulating Mouse Mesangial Cell Functions in a Glucose-Induction Cell Model
Figure 3
Change of cell contraction, F/G-actin ratio, and arrangement of F-actin in the CCT2 cell model with and without glucose stimulation. Four designated conditions varying in culture medium containing CCT2 or scramble siRNA were used to study the changes of the planar areas of mMC in response to 1 μM PMA stimulation. Representative plots show (a) the morphological changes of mMC (magnification 250x) before (0 min) and after (60 min) PMA treatment and (b) the degrees of cell contraction recorded at 10-min intervals. As group CsiRNA pN exhibited the highest contractility with 40–50% planar area reduction at 60 min, the other groups disclosed lower contractility 30 min after PMA stimulation, whereas n denotes the number of cells and * represents versus CsiRNA pN. Changes in actin were studied in the same conditions. Representative pictures show (c) the immunohistochemical blotting of actin in mMC with highlighting F-actin (green) and nuclei (blue) and (d) the change of F/G-actin ratio before and after PMA stimulation, in which a similar pattern persisted for the four groups.
