Involvement of F-Actin in Chaperonin-Containing t-Complex 1 Beta Regulating Mouse Mesangial Cell Functions in a Glucose-Induction Cell Model

Figure 4

Change of cell proliferation, migration, F/G-actin ratio, and arrangement of F-actin in CCT2 cell model with and without high glucose stimulation. The same four designated groups in previous experiments were studied with high and normal glucose cultured for 24 hrs. Then, wound healing assay was performed to evaluate mMC migration. Representative pictures show (a) the images of mMC migration to close the wound, (b) the rate of migration, and (c) the rate of proliferation during the 12 hr testing interval. Group CsiRNA pH exhibited the highest migration activity among these groups. These experiments were performed in triplicate, and the rate of migration activity was calculated as %  = (area at 0 hr–area at 12 hr)/area at 0 hr. Changes in actin were also studied. Representative pictures show (d) the immunohistochemical blotting of actin with highlighting F-actin (green) and nuclei (blue) and (e) the change of F/G-actin ratio during the migration testing interval. As seen, a similar pattern of no significant change was observed across the four groups.