Targeted Proteomics of Isolated Glomeruli from the Kidneys of Diabetic Rats: Sorbin and SH3 Domain Containing 2 Is a Novel Protein Associated with Diabetic Nephropathy

Table 2

Differentially expressed actin cytoskeleton-associated proteins in glomeruli isolated from OLETF and LETO rats, identified by QSTAR Elite LC-MS/MS and IPA.

Protein	GI number	Mass (Da)	Location	Function	Foldchange	𝑃 value	Fold change	𝑃 value
					(27 weeks)		(38 weeks)
Stress fiber formation
Sorbin and SH3 domain containing 2 (SORBS2)	205831248	124108	C	AD	1.49	<0.0001	1.97	<0.0001
Alpha-actinin 1 (ACTN1)	13123942	103058	C	ST, CL	1.02	0.764	1.40	<0.0001
Alpha-actinin 4 (ACTN4)	182705246	104654	C	ST, CL	0.93	0.46	1.22	<0.0001
Rho GDP dissociation-inhibitor alpha (ARHGDIA)	21759130	23207	C	RI	1.28	0.041	0.87	0.0413
Actin-filament polymerization
Actin-related protein 2/3 complex subunit 1 beta (ARPC1B)	12229626	40950	C	AP, EC	0.65	0.0363	0.73	0.0136
Actin related protein 2/3 complex subunit 5 (ARPC5)	3121767	16320	C	AP, EC	0.72	0.0014	0.74	0.0002
Myristoylated alanine-rich protein kinase C substrate (MARCKS)	266495	31555	UN	AP	0.60	0.0004	0.71	0.002
Microtubules formation
Tubulin alpha 1c (TUBA1C)	55976169	49895	C	MT	0.74	0.012	0.90	0.17
Intermediate filaments formation
Lamin A/C (LMNA)	1346413	74139	N	IF	0.89	0.0076	1.29	<0.0001
Desmin (DES)	1352241	53536	C	IF	0.94	0.143	1.32	<0.0001
Nestin (NES)	146345465	177439	C	IF	0.83	<0.0001	1.03	0.32
Plectin 1 (PLEC1)	1709655	531791	C	IL	0.82	<0.0001	0.96	0.12
GBM (glomerular basement membrane)
Integrin beta 1 (INTGB1)	1352494	15105	PM	APG	0.82	0.0015	0.92	0.0094
Agrin (AGRN)	399021	214846	PM	LB	0.62	0.042	0.90	0.490
Others
Plastin 3 (PLS3)	226693553	70811	C	CL	0.82	0.02	0.82	0.0005
Calponin 3 (CNN3)	584956	36414	C	AB	0.60	0.0013	0.78	0.088
Tropomyosin 3 (TPM3)	148840439	32819	C	AS	0.71	<0.0001	0.80	<0.0001

AB: actin binding; AD: adaptor protein; AP: actin filament polymerization; APG: anchoring podocyte and GBM (glomerular basement membrane); AS: actin filaments stabilization; C: cytoplasm; CL: crosslinking actin filaments into bundles or networks; EC: endocytosis; IF: intermediate filaments; IL: interlinks; LB: laminin binding; LETO: Long-Evans Tokushima Lean; MT: microtubules; N: nuclear; OLETF: Otsuka Long-Evans Tokushima Fatty; PM: plasma membrane; RI: Rho GDP-dissociation-inhibitor activity; ST: stress fibers; UN: unknown.