Time-Dependent Alterations in Rat Macrovessels with Type 1 Diabetes

Figure 9

Extracellular matrix. (a) The elastic band width, measured from EM sections, was significantly higher in all diabetic samples (^∗𝑃<0.001) although it followed the control pattern of decline with age (^#𝑃<0.05; 𝑛=80 measurements from 6 rats/group for 50 day, 𝑛=100 measurements from 3 rats/group for 100 day, and 𝑛=60 measurements from 3 rats/group for 150 day). (b) A representative zymography is shown. Zymography of control and diabetic aortic proteins revealed decreased MMP activity with diabetes. Bacterial collagenase (5 ng/lane) was used as a positive control (P) for zymography. Molecular weight markers are indicated to the left. (c) Zymogram quantification revealed a statistically significant decline in MMP activity. (𝑛=3/group). The band included in the analysis is the most intense, as indicated with the asterisk.

(a)

(b)

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