Suppression of the Nuclear Factor Eny2 Increases Insulin Secretion in Poorly Functioning INS-1E Insulinoma Cells

Figure 2

GSIS by INS-1E cells after overexpression of Eny2 and siRNA-mediated Eny2 knockdown. (a) Mouse Eny2 was transiently overexpressed in INS-1E cells by plasmid transfection. No change of glucose and exendin-4-induced insulin secretion was observed 72 hours after transfection (𝑛=3). (b) Glucose-dependent insulin secretion was increased 72–96 hours after transfection with two different Eny2-specific siRNAs compared to a control siRNA (𝑛=5). *𝑃<0.05, **𝑃<0.01, ***𝑃<0.001; the unpaired 𝑡-test was used for comparisons between two experimental conditions, one-way ANOVA with posttests when 3 or more conditions were analyzed.

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