Research Article

Ca+2/Calmodulin-Dependent Protein Kinase Mediates Glucose Toxicity-Induced Cardiomyocyte Contractile Dysfunction

Figure 4

Intracellular Ca2+ handling property of adult rat cardiomyocytes cultured for 12 hours in a serum-free medium with normal glucose (NG: 5.5 mM) or high glucose (HG: 25.5 mM) in the absence or presence of high extracellular Ca2+ (Hi-Ca, 2.7 mM) in the contractile buffer. An extracellular Ca2+ concentration of 1.0 mM was used as the low Ca2+ (LoCa) environment. (a) Resting intracellular Ca2+ levels shown as baseline fura-2 fluorescent intensity (FFI), (b) peak FFI, (c) electrically stimulated rise of FFI (ΔFFI), and (d) intracellular Ca2+ decay rate. Mean ± SEM, –42 cells per group, * versus NG-LoCa group, # versus HG-LoCa group.
829758.fig.004a
(a)
829758.fig.004b
(b)
829758.fig.004c
(c)
829758.fig.004d
(d)