Functional Expression of TRPV4 Channels in Human Collecting Duct Cells: Implications for Secondary Hypertension in Diabetic Nephropathy

Figure 3

The effect of glucose on TRPV4 and SGK1 protein expressions in HCD cells. HCD-cells were incubated in 5 mM and 25 mM glucose for 48 hours. (a) Representative Western blot analysis using an anti-TRPV4 antibody; (c) analysis of changes in TRPV4 protein expression. Results represent mean ± SEM; 𝑛=4; **𝑃<0.01. (b) Representative Western blot analysis using an anti-SGK antibody; (d) analysis of changes in SGK protein expression. Results represent mean ± SEM; 𝑛=4; **𝑃<0.01.

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