Functional Expression of TRPV4 Channels in Human Collecting Duct Cells: Implications for Secondary Hypertension in Diabetic Nephropathy

Table 1

Polymerase chain reaction (PCR) primers used to amplify TRPV4 mRNA. F: forward primer, R: reverse primer.

mRNA	Primers (5′→3′)	Direction	Annealing temp	Product size
TRPV4	CCCGTGAGAACACCAAGTTT; TCACTCCAGGGCATTTCTTC	F; R	58°C	618 bp