BlockingαVβ3 Integrin Ligand Occupancy Inhibits the Progression of Albuminuria in Diabetic Rats

Figure 1

Biotin labeled C-loop antibody binding to β3 in rat kidney lysates from two diabetic rats (R1 and R2) as well as lysate from CHO cells (that do not express β3) was immunoprecipitated (IP) with an anti-αV antibody and then immunoblotted (IB) with a biotin labeled C-loop antibody (C-loop biotin). RIPA buffer alone was used as an additional negative control (−). Binding of the C-loop antibody to β3 in the kidney lysate but not to the CHO lysate was visualized with avidin HRP and enhanced chemiluminescence. To demonstrate that the specific band recognized by the C-loop biotin antibody corresponded to β3, lysates were also immunoblotted with an anti-β3 antibody.