Research Article

Pituitary Adenylate Cyclase-Activating Polypeptide Protects Glomerular Podocytes from Inflammatory Injuries

Figure 4

Effects of PACAP on the expression of inflammatory cytokines and TLR-related genes in podocytes. Podocytes were pretreated with 10 nM PACAP for 1 h and then stimulated with 0.1 μg/mL LPS for 3 h. The expressions of MCP-1, IL-6, TLR4, TLR2, MyD88, and IRAK1 mRNA were analyzed by real-time PCR. GAPDH was used as an internal standard. compared with the control group. compared with the LPS group. Experiments were repeated at least three times and bars represented mean ± SD.
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