Research Article

Pituitary Adenylate Cyclase-Activating Polypeptide Protects Glomerular Podocytes from Inflammatory Injuries

Figure 5

Effect of PKA inhibitor and PLC inhibitor on the expression of MCP-1 induced by LPS. Podocytes were pretreated with PACAP with or without 10 mM H89 (PKA inhibitor) or U-73122 (PLC inhibitor) for 1 h and then stimulated with 0.1 μg/mL LPS for 3 h. The expression of MCP-1 mRNA was analyzed by RT-PCR. GAPDH was used as internal standard. indicated . Experiments were repeated at least three times and bars represented mean ± SD.