A Combination of Human Embryonic Stem Cell-Derived Pancreatic Endoderm Transplant with LDHA-Repressing miRNA Can Attenuate High-Fat Diet Induced Type II Diabetes in Mice

Figure 1

Sequence alignment of hsa-miR-590-3p and LDHA mRNA and confirmation of inhibitory function of hsa-miR-590-3p on LDHA using luciferase assay and HeLa cells. (a) Sequence alignment of hsa-miR-590-3p and LDHA mRNA. (b) The construct for luciferase assay. (c) The relative activity of GLuc/SeAP in luciferase assay. (d) Real-time qPCR results of LDHA expression in HeLa cells transfected with no miR, miR-590, and miR-NC. (e) Western blot results for LHDA protein in HeLa cells transfected with no miR, miR-590, and miR-NC. All values are given as mean; error bar represents standard error. .

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