Adipose Tissue-Derived Mesenchymal Stem Cells Exert In Vitro Immunomodulatory and Beta Cell Protective Functions in Streptozotocin-Induced Diabetic Mice Model

Figure 2

Proliferation index of splenocytes from normal (a) and diabetic (b) mice cocultured with AT-MSCs in presence of islet cells lysate as specific stimulator. Diabetic splenocytes showed a sharp proliferation in the presence of lysate, in which normal splenocytes remained nonproliferated. AT-MSCs caused cell death of some resting splenocytes in the absence of lysate (proliferation index < 1.0). AT-MSCs could suppress diabetic splenocytes proliferation in response to lysate in a dose-dependent manner, where higher concentration of AT-MSCs induced cell death in some splenocytes (proliferation index < 1.0). Each bar represents the average of five independent experiments. indicates significant difference between groups (). Lysate: pancreatic islet cells lysate; SP: splenocytes.

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