JNK1 Deficient Insulin-Producing Cells Are Protected against Interleukin-1β-Induced Apoptosis Associated with Abrogated Myc Expression

Figure 4

JNK1 knockdown attenuates IL-1β-induced caspase 3 activation and apoptosis in INS-1 cells. Stable INS-1 cell lines expressing shRNA for JNK1, JNK2, JNK3, nonsense shRNA, or empty vector controls were exposed to 150 pg/mL IL-1β (closed bars) or vehicle (open bars) for 24 h. (a) Apoptosis was measured as the relative levels of cytoplasmic nucleosomes in INS-1 stable cell lines lysates using the Roche Cell Death detection Elisa kit. Data are shown as means ± SEM of independent experiments. (b) Cleaved caspase 3 was assessed with immunoblotting and normalized to actin. Data are shown as means ± SEM of independent experiments; , , and , comparing caspase 3 activity in the presence with the activity in the absence of IL-1β, unless otherwise indicated. Representative gels are shown.

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