ALDH2 Inhibition Potentiates High Glucose Stress-Induced Injury in Cultured Cardiomyocytes

Figure 4

Disulfiram (DSF) pretreatment and cell death. (a) Representative Hoffman modulation-contrast microscopic images of cells were shown. The normal cell morphology was maintained in mannitol with no DSF. However, the cells from all other 3 groups were depicting cell death (shape altered with cell damage) from all 4 groups in various degrees. In the HG and DSF group, lot of cells show cell disintegration depicting cell death. (b) DSF 10 μM treatment leads to cell death cultured H9C2 cardiomyocytes subjected to high glucose stress (HG) compared to equimolar mannitol (Ctrl). Trypan exclusion assay was used to measure the cell death. The data expressed are mean ± SEM. = 4–6. G1, G2, G3, and G4 depict mannitol, high glucose stress, DSF + mannitol, and DSF + high glucose stress, respectively. versus G1, versus G2, and versus G3.

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