Influence of Acute High Glucose on Protein Abundance Changes in Murine Glomerular Mesangial Cells

Figure 4

2DE immunoblot experiments were used to determine the effects of HG and on PHB isoforms. Following the transfer and development of PHB IB, images were aligned and densitometric measurements were estimated using ImageJ for the acidic one-third of the PHB charge train and for the basic two-thirds of the PHB charge train (IB images on right). Fractional values for PHB charge train components (acid and basic ends) were used to determine statistical significance differences (left bar graphs). M is the same as (5 mM D-glucose + mannitol); HG is 25 mM D-glucose. value < 0.01.