Uninephrectomized High-Fat-Fed Nicotinamide-Streptozotocin-Induced Diabetic Rats: A Model for the Investigation of Diabetic Nephropathy in Type 2 Diabetes

Figure 1

Schematic diagram of the experimental protocol. NE: right-side nephrectomy; STZ: streptozotocin; NA: nicotinamide; MA: light microscopy and morphometric analysis; IHC: immunohistochemistry; EM: electron microscopy; i.p.: intraperitoneally; OGTT: oral glucose tolerance test. NA-STZ group: uninephrectomized rats received the high-fat diet containing beef tallow for 5 weeks (from week 3 till week 8), and then successive intraperitoneal injections of NA (230 mg/kg) and STZ (65 mg/kg) in 15 min interval were administered. LD-STZ group: uninephrectomized rats received the same high-fat diet at the same period of the experiment, and then intraperitoneal injection of STZ in a lower dose (40 mg/kg) was administered. Control group: uninephrectomized rats received the regular commercial rodent chow during the experiment and received vehicle instead of STZ/NA-STZ.