Uninephrectomized High-Fat-Fed Nicotinamide-Streptozotocin-Induced Diabetic Rats: A Model for the Investigation of Diabetic Nephropathy in Type 2 Diabetes

Figure 12

Tubulointerstitial lesions in experimental groups at week 30. (a) Representative immunostaining with anti-collagen type IV antibodies (upper row, original magnification ×200) and Masson’s trichrome staining (lower row, original magnification ×320) of kidney sections from control nondiabetic group, low-dose streptozotocin-injected group, and nicotinamide-streptozotocin-induced diabetic group. Kidney tissues from both diabetic groups demonstrate tubulointerstitial lesions with tubular dilation and partial tubular atrophy compared to control nondiabetic rats and are characterized by interstitial inflammatory infiltration (indicated by white arrows on Figure 11(a)). Marked increase expression of collagen type IV is seen in tubular basement membrane, vascular walls, and interstitial areas in both diabetic groups. (b) Graphic representation of semiquantitative indices of tubulointerstitial fibrosis in control and two diabetic groups. Mean values and standard deviations are shown.

(a)

(b)