Hyperglycemia Does Not Affect Iron Mediated Toxicity of Cultured Endothelial and Renal Tubular Epithelial Cells: Influence of L-Carnosine

Figure 4

Influence of glucose and L-carnosine on DMT1 (a) and TFRC (b) mRNA expression. HUVECs (open bars) and PTECs (filled bars) were cultured for 24 h in the presence of 20 mM L-carnosine (CN), 60 μM of FeCl₃, or both combinations. In addition cells were cultured for 3 days in HG medium (Glc). Total RNA was isolated and DMT1 (a) and TFRC (b) mRNA expression were assessed by qPCR. In (c) protein expression of DMT1 in HUVECs and PTECs is shown.

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