Clopidogrel Protects Endothelium by Hindering TNFα-Induced VCAM-1 Expression through CaMKKβ/AMPK/Nrf2 Pathway

Figure 4

Nrf2 are implicated in HO-1 expression induced by INN. (a) ECs were treated with 10 μM Compound C for 1 h followed by incubation with 10 μM INN for the indicated time. Aliquots of cell lysate underwent immunoblotting analysis. versus control; versus INN (24 h). (b) ECs were transfected with shLuc or shNrf2 and then treated with 10 μM INN for 16 h. Aliquots of cell lysate underwent immunoblotting analysis. (c) Total RNA was extracted from ECs and was used to analyze HO-1 and β-actin mRNA by using RT-PCR with their specific primers. Data are expressed as means ± SD (). versus control; versus INN/shLuc.

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