Hepatic Cholesterol-25-Hydroxylase Overexpression Improves Systemic Insulin Sensitivity in Mice

Figure 5

Overexpression of hepatic Ch25h improved glucose tolerance, insulin sensitivity, and lowered HOMA-IR. (a) Oral glucose tolerance tests (OGTT) performed in AdLacZ and AdCh25h transduced C57BL/6J animals. The glucose (mg/dl) and insulin (ng/ml) levels were measured 15, 30, 45, and 60 min after administering glucose solution (1 g/kg). Blood glucose and insulin levels were significantly lower in the AdCh25h transduced subpopulation. Two-sided values obtained from unpaired -test. Results are mean ± SEM. , , and . per group. (b) Insulin tolerance tests (ITTs) on AdCh25h and AdLacZ transduced C57BL/6J mice. Regular human insulin was administered intraperitoneally and blood glucose levels were monitored and measured 15, 30, 45, and 60 min after injections. The blood glucose levels were clearly decreased in mice treated with AdCh25h. Two-sided values obtained from unpaired -test. Results are mean ± SEM. , per group. (c) HOMA-IR levels of AdLacZ and AdCh25h transduced C57BL/6J mice. Comparison of the control group to the AdCh25h treated mice showed a significant decrease in HOMA-IR levels. Results are mean ± SEM. , per group.

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