L-Carnitine: An Antioxidant Remedy for the Survival of Cardiomyocytes under Hyperglycemic Condition

Figure 2

Attenuation of ROS production induced by H₂O₂ in LC-pretreated H9c2 cells. (a) Scheme of treatment: H9c2 cardiomyocytes were exposed to a stressful insult with 500 μM of H₂O₂ after pretreatment for 48 h with 0.5 or 5 mM LC. (b) Using the CellROX reagent assay, LC action was evaluated under oxidative stress conditions. The pretreatment with 0.5 or 5 mM LC causes a reduction in ROS production after stimulus with 500 μM of H₂O₂. (c) Quantification of ROS production. The quantified signal was normalized for the total nuclei number. From the DAPI images, it was possible to observe that the cells pretreated with both doses of LC show higher survival compared to the control cells. (d) Quantification of nuclei number pre- and after H₂O₂ injury in LC-pretreated H9c2 cells. Data are expressed as fold changes (FC) of mean ± SD. Significance: vs. CONTR.

(a)

(b)

(c)

(d)