Novel Mechanisms Modulating Palmitate-Induced Inflammatory Factors in Hypertrophied 3T3-L1 Adipocytes by AMPK

Figure 1

Effects of AICAR on the mRNA expressions and release of MCP-1 in 24 h palmitate-preloaded 3T3-L1 adipocytes. Differentiated 3T3-L1 adipocytes were preincubated with 0.3–1.0 mmol/L of AICAR (a) for 1 h and then treated with 0.3 mmol/L palmitate (Pa) (black bar) or ethanol vehicle alone (white bar) for 24 h. AMPK phosphorylation of Thr172 (a) and intracellular MCP-1 (c) was quantified by immunoblot analysis, and MCP-1 release was also assessed by ELISA (d). The mRNA levels of MCP-1 (b) were measured by quantitative real-time RT-PCR at 12 h after stimulation and then normalized over the 18S rRNA signal. Data are means ± SEM (). , compared to corresponding control cells.

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