Novel Mechanisms Modulating Palmitate-Induced Inflammatory Factors in Hypertrophied 3T3-L1 Adipocytes by AMPK

Figure 3

Effects of AICAR on intracellular TG contents in 24 h palmitate-preloaded 3T3-L1 adipocytes. Differentiated 3T3-L1 adipocytes were exposed to 0.3 mmol/L palmitate (black bar) or ethanol vehicle alone (white bar) in the presence or absence of 0.5 mmol/L AICAR for 24 h. (a) Cellular TG contents were measured, and the concentrations were then adjusted to intracellular total protein contents. (b) Lipid drops were stained with oil red O and examined using fluorescence microscope. (c) ACC phosphorylation on Ser79 was then quantified by immunoblot analysis. Data are means ± SEM (). compared to corresponding control cells.

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