In Silico Analysis of Coding/Noncoding SNPs of Human RETN Gene and Characterization of Their Impact on Resistin Stability and Structure

Table 2

Details of nsSNPs selected as deleterious among the reported SNPs, their conservation analysis by ConSurf, and posttranslational modification site prediction by ModPred.

Position	Conservation score	B/E	F/S	PTM sites (wild type)	Variants	PTM sites (mutants)
I32	7	B	—	—	I32S	—
C51	9	B	S	—	C51Y	Amidation; Phosphorylation
G58	9	E	F	Proteolytic cleavage	G58R	Proteolytic cleavage
					G58E	Proteolytic cleavage
G66	7	E	—	Proteolytic cleavage	G66R	Proteolytic cleavage
G71	4	B	—	—	G71R	Proteolytic cleavage; ADP-ribosylation
C74	9	B	S	Disulfide linkage	C74W	—
C78	9	B	S	Disulfide linkage	C78S	O-linked glycosylation
G79	9	E	F	—	G79C	Disulfide linkage
R84	6	E	—	ADP-ribosylation; Proteolytic cleavage	R84C	Disulfide linkage
D97	9	E	F	—	D97E	Disulfide linkage
W98	8	B	—	C-linked glycosylation; Proteolytic cleavage; Amidation	W98C	Disulfide linkage
					W98L	—
C103	9	B	S	Disulfide linkage	C103	Proteolytic cleavage
C104	9	B	S	Disulfide linkage	C104	Amidation; Proteolytic cleavage

PTM: posttranslational modification; B: buried; E: exposed; F: functional; S: structural.