FOXO1 Mediates Advanced Glycation End Products Induced Mouse Osteocyte-Like MLO-Y4 Cell Apoptosis and Dysfunctions

Figure 3

AGE enhances FOXO1 expression and promotes FOXO1 nuclei localization. Western blot was performed to examine the expression of Akt and p-Akt. (a) Akt and p-Akt representative images. (b) Quantifications of p-Akt expression. Western blot was performed to examine the expression of FOXO1. (c) FOXO1 representative images. (d) Quantifications of FOXO1-relative expression. Immunofluorescence pictures taken with FOXO1 antibody to detect FOXO1 nuclei localization, images were taken at 40 for FOXO1-positive cell counting and 100 for exhibiting nuclei localization. (e) Immunofluorescence representative images for FOXO1 nuclei translocation. (f) Percentage of FOXO1 nuclei-positive cells was set by the number of FOXO1 nuclei-positive cells divided by the total number of FOXO1-positive cells. All error bars are standard deviation of the mean. compared to the BSA control group.

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