Effects of BSF on Podocyte Apoptosis via Regulating the ROS-Mediated PI3K/AKT Pathway in DN

Figure 3

Effects of BSF on the ROS-mediated PI3K/AKT pathway in DN mice and HG-cultured podocytes. (a) Representative NOX-4 protein band in the renal cortex from mice in different groups. (b) Comparison of NOX-4 protein in the mouse renal cortex (). (c) Comparison of NOX-4 mRNA levels in the rat renal cortex (). (d) Representative NOX-4 protein bands from cultured podocytes in different groups. (e) Comparison of NOX-4 protein from cultured podocytes (). (f) Comparison of NOX-4 mRNA levels in cultured podocytes (). (g) Representative photograph of PI3K and P-AKT staining in cultured podocytes. (h) Comparison of ROS production in cultured podocytes from different groups. (i) Representative PI3K, P-PI3K, AKT, and P-AKT protein bands in the renal cortex of mice in different groups. (j) Comparison of P-PI3K protein in the mouse renal cortex (). (k) Comparison of P-AKT protein in the mouse renal cortex (). (l) Representative PI3K, P-PI3K, AKT, and P-AKT protein bands in cultured podocytes in different groups. (m) Comparison of P-PI3K protein in cultured podocytes (). (n) Comparison of P-AKT protein in cultured podocytes ().

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