The Diethylcarbamazine Delays and Decreases the NETosis of Polymorphonuclear Cells of Humans with DM Type 2

Figure 2

Morphological analysis of NETosis shown by PMNs from the healthy volunteer group. Photocomposition of confocal microphotographs of PMNs stained with indirect immunofluorescence technique with rabbit monoclonal anti-NE antibodies and mouse polyclonal anti-rabbit IgG antibodies conjugated with Alexa Fluor 488 (green color) and DAPI (blue color). PMA (positive control), DEC+PMA (experimental group), and RPMI (negative control) at 0, 30, 60, 120, and 180 minutes post challenge with PMA. In the positive control group, functional profiles of PMNs are present from 0 till the 15^th minute, some activated PMNs with rounded aspect (arrows) appear at the 30^th minute, and numerous NETs (asterisks) with reticular appearance can be seen from the 60^th minute to the end. In the experimental group, functional profiles of PMNs are present from 0 till the 30^th minute, and just some PMNs with morphological data of activation (arrows) are present from the 60^th till the 180^th minute. In the negative control group, all profiles of PMNs have a morphological aspect of functional cells along the studied times. Multiphoton confocal microscopy, 63x; lasers: 405 and 488 nm.