The Diethylcarbamazine Delays and Decreases the NETosis of Polymorphonuclear Cells of Humans with DM Type 2

Figure 3

Morphological analysis of NETosis shown by PMNs from the diabetes mellitus volunteer group. Photocomposition of confocal microphotographs of PMNs stained with indirect immunofluorescence technique with rabbit monoclonal anti NE antibodies and mouse polyclonal anti-rabbit IgG antibodies conjugated with Alexa Fluor 488 (green color) and DAPI (blue color). PMA (positive control), DEC+PMA (experimental group), and RPMI (negative control) at 0, 30, 60, 120, and 180 minutes post challenge with PMA. In the positive control group, the PMNs show the morphological aspect of activation (arrows) at the 30^th minute and NETosis (asterisks) is observed from the 60^th minute till the end. In the experimental group, PMNs show morphological data of activation at the 60^th minute and NETs can be observed till the end of the experiment. Finally, in the negative control group, the cells show morphological characteristics of functionality in all studied times. Multiphoton confocal microscopy, 63x; lasers: 405 and 488 nm.