Abnormal Peripheral Neutrophil Transcriptome in Newly Diagnosed Type 2 Diabetes Patients

Figure 6

RT-qPCR validation of the RNA-seq results for a subset of 12 genes. In order to validate the RNA-seq transcriptome, 12 genes were selected from diverse biological functional categories, and RT-qPCR was performed on these genes. External samples including type 2 diabetes patients () and healthy controls () were selected for RNA-qPCR validation. Blue bars denote the RNA-seq fold induction values, while colored bars represent RT-qPCR fold induction values calculated using the 2^-ΔΔCt method, and data are presented as the . For RNA-qPCR validation, CXCR1, SELL, SELP, and S100A8 were significantly increased in neutrophils from type 2 diabetes patients compared with healthy controls ().