Impact of PCSK9 Immunization on Glycemic Indices in Diabetic Rats

Figure 2

The function of liposomal anti-PCSK9 vaccine in rats upon 4 vaccinations. (a) Anti-PCSK9 peptide IgG titers () in vaccinated and control rats (/group). (b) Concentrations of the plasma PCSK9 in vaccinated and control rats were and , respectively ( replicates of the pooled samples of 8 rats per group). (c) Direct detection of vaccine-generated anti-PCSK9 antibodies targeting plasma PCSK9. Increased OD₄₅₀ is indicative for evaluating the direct binding of anti-PCSK9 antibodies to plasma PCSK9 from vaccinated and control rats ( replicates of the pooled samples of 8 rats per group). (d) In vitro evaluation of PCSK9/LDLR interaction. Plasma sample of vaccine rats contained vaccine-generated anti-PCSK9 antibodies that could decrease PCSK9 interaction to LDLR by 30% when compared with the plasma sample of control rats ( replicates of the pooled samples of 8 rats per group). Bars show mean values, error bars show ±SD.

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