Inhibition of miRNA-155 Alleviates High Glucose-Induced Podocyte Inflammation by Targeting SIRT1 in Diabetic Mice

Figure 4

The effects of miR-155 silencing on podocyte inflammation was reversed by SIRT1 downregulation. (a, b) SIRT1 expression in podocytes transfected with the miR-155 mimic or inhibitor under high glucose conditions was evaluated using real-time PCR and Western blotting analysis. ^#Compared with control group, ; Compared with HG (high glucose) group, . (c, d) The expression of SIRT1 in podocytes transfected with the miR-155 inhibitor and SIRT1 siRNA under high glucose conditions was assessed using real-time PCR and Western blotting analysis. ^#Compared with HG (high glucose) group, ; Compared with miR-155 inhibitor + HG group, , the same below. (e–h) Inflammatory cytokine expressions (MCP-1, TNF-α, IL-1β, and IL-6) in podocytes transfected with the miR-155 inhibitor and SIRT1 siRNA under high glucose conditions was determined using ELISA. (i) Nephrin expression in podocytes cotransfected with the miR-155 inhibitor and SIRT1 siRNA under high glucose conditions was quantified using Western blotting analysis.

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