ROS-ERK Pathway as Dual Mediators of Cellular Injury and Autophagy-Associated Adaptive Response in Urinary Protein-Irritated Renal Tubular Epithelial Cells

Figure 1

Effect of antioxidants on urinary protein-induced ERK pathway activation. Antioxidants NAC (10 mM) and CAT (2000 U/ml) were administered 1 h before the addition of 8 mg/ml urinary proteins for 2 h. Cell culture supernatant was subjected to DCFH-DA flow cytometry analysis of ROS levels (a, b). After treatment with urinary proteins for 4 h, aliquots of cell lysate were subjected to Western blot analysis of p-ERK and t-ERK level (c, d). (b) ; (d) . and . CON: control; NAC: N-acetylcysteine; CAT: catalase; UP: urinary proteins.

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