ROS-ERK Pathway as Dual Mediators of Cellular Injury and Autophagy-Associated Adaptive Response in Urinary Protein-Irritated Renal Tubular Epithelial Cells

Figure 2

Effect of MEK inhibitor on urinary protein-induced injury. MEK inhibitor U0126 (10 μM) was administered 1 h before the addition of 8 g/l urinary proteins. The aliquots of cell lysate were subjected to Western blot analysis of p-ERK and t-ERK levels (a, b), and cellular apoptosis was assessed by Annexin V-FITC/PI flow cytometry analysis (c, d) at 48 h. Cell culture supernatant was subjected to ELISA analysis of the NGAL and KIM-1 secretion at 12 h (e, f). (b) ; (d) ; (e) ; (f) . , , and . CON: control; UP: urinary proteins.

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