ROS-ERK Pathway as Dual Mediators of Cellular Injury and Autophagy-Associated Adaptive Response in Urinary Protein-Irritated Renal Tubular Epithelial Cells

Figure 3

Effect of MEK inhibitor on urinary protein-induced autophagy. MEK inhibitor U0126 (10 μM) was administered 1 h before the addition of 8 g/l urinary proteins for 8 h. Aliquots of cell lysate were subjected to Western blot analysis of p-ERK, t-ERK, LC3, and p62 levels. (b) ; (c) . . CON: control; UP: urinary proteins.

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