Research Article
Bradykinin Protects Human Endothelial Progenitor Cells from High-Glucose-Induced Senescence through B2 Receptor-Mediated Activation of the Akt/eNOS Signalling Pathway
Figure 3
Effects of BK pretreatment on HG-induced EPC senescence. (a) Representative photomicrographs of SA-β-gal staining in EPCs. (c) Number of senescent cells after treatment. (b, d) ROS generation was analysed by Mito-Sox. (e) Cell viability was evaluated by CCK-8 assay. (f) NO production was detected by NO assay kit. (g) The mRNA expression of p16, p21, and p53 was detected by real-time PCR. (h, i) Western blotting for p16, p21, and p53 in EPCs. Bars represent the ( versus control; ).
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