Research Article
miR-126 Mimic Counteracts the Increased Secretion of VEGF-A Induced by High Glucose in ARPE-19 Cells
Figure 3
Expression of SPRED1 in ARPE-19 cells cultured for 24 hours in standard medium (CTR), silencing (inhib) and upregulating (mimic) miR-126 in control condition and in presence of high glucose (HG). (a) SPRED1 gene expression normalized vs. β-actin as housekeeping gene. (b) Representative Western blot analysis of SPRED1 protein expression with quantification of densitometries of relative bands. Data were expressed as of fold induction relative to β-actin of three independent experiments (). , , and vs. CTR; § and HG+mimic vs. HG.
(a) |
(b) |