Low Molecular Weight Fucoidan Can Inhibit the Fibrosis of Diabetic Kidneys by Regulating the Kidney Lipid Metabolism

Figure 4

Assessment of lipid metabolism in HK-2 cells. (a) Observing the state of HK-2 cells. (b) Cytotoxicity of LMWF effect on HK-2 cells. (c) LMWF concentration screening ( vs. the HG + PA group; ## vs. the NG group, repeat three times). (d) Detection of fibrosis biomarkers and regulatory factor protein expressions by western blot. (e) Statistical protein expressions of fibrosis biomarkers and the regulatory factors of lipid metabolism ( vs. the HG + PA group; ## vs. the NG group; &, && vs. the HG group, repeat three times). (f) Observing the state of HBZY-1 cells. (g) Cytotoxicity of LMWF effect on HBZY-1 cells. (h) LMWF concentration screening ( vs. the HG + PA group; ## vs. the NG group, repeat three times). (i) TC and (j) TG contents in the HBZY-1 cells (^## vs. the Sham group; vs. the DKD group, ). (k) Detection of fibrosis biomarkers and regulatory factor protein expressions in HBZY-1 cells by western blot. (l) Statistical protein expressions of fibrosis biomarkers and the regulatory factors of lipid metabolism ( vs. the HG + PA group; #, ## vs. the NG group; &, && vs. the HG group, repeat three times). The statistical results were compared with the Sham operation group and other groups.

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