Islet Transplantation Reverses Podocyte Injury in Diabetic Nephropathy or Induced by High Glucose via Inhibiting RhoA/ROCK/NF-κB Signaling Pathway

Figure 4

The effects of islet transplantation on synaptopodin and RhoA/ROCK1 proteins in diabetic nephropathy mice. (a, b) Synaptopodin protein was detected by immunohistochemical staining (×200, ×400), and then, podocyte injury and restoration were quantitatively analyzed in different groups. Quantifications of Synaptopodin protein expression in single glomeruli was measured by mean integrated optical density (IOD)/area. (c, d) Western blot was used to detect the expression of synaptopodin protein and quantitatively analyze recovery of podocytes after islet transplantation. (e, f) The expression and distribution of RhoA/ROCK1 proteins in immunohistochemical photographs (×200, ×400) of different kidney tissues. (g) Quantifications of RhoA/ROCK1 proteins in single glomeruli was measured by mean integrated optical density (IOD)/area. (h, i) Western blot and semiquantitative analysis of RhoA/ROCK1 proteins expression in renal tissue ( versus the control group, ^# versus the DN group).

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