An In Vitro Model of Diabetic Retinal Vascular Endothelial Dysfunction and Neuroretinal Degeneration

Figure 2

Cell migration ability detected by Transwell assay. (a) Representative images of migrated HRMECs treated with 5.5 mM, 25 mM, 50 mM, and 100 mM glucose, respectively, at 48 h. HRMEC cells were stained with 0.1% crystal violet (yellow, arrows). (b) The number of migrated HRMEC was significant higher in the 25 mM ( vs. , ), 50 mM ( vs. , ), and 100 mM ( vs. , ) groups in comparison with the 5.5 mM groups. Scale bar: 200 μm (a). HRMEC: human retinal vascular endothelial cells; Con: control group (5.5 mmol/l); G25: 25 mmol/l; G50: 50 mmol/l; G100: 100 mmol/l. .

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